Lyme Disease (Borrelia burgdorferi B31)
Line Immunoassays

fda_cleared

GSD offers both a highly specific line immunoassay (LIA) confirmation test and sensitive ELISA screen to fulfill the CDC’s recommended Lyme disease diagnostic process. The LIA offers numerous improvements compared to traditional Western blot technology.

Line Immunoassay (LIA) vs. Western Blots

Line Immunoassay strips offer easy handling, simple procedures and intuitive interpretation. Results can be read by virtually anyone with minimal training and experience.

Traditional “Western Blots” are not intuitive to read and require a Band Locator to interpret results.

Line Immunoassay (LIA) Advantages

  • Discrete Antigen Application – Antigen applied in tightly defined, discrete positions on each strip.
  • Native Antigen Structure – The 3D (tertiary) structure is maintained for LIA. Western blot proteins are denatured, and only their primary structure remains intact. Antibodies bond with proteins in their native structure.
  • Intuitive Results – Easy-to-read, precisely defined results. No special Band Locater or subjective judgment required.
  • Easy Handling – Strips fixed in a numbered booklet (32 strips per booklet).
  • Durable Strips – Strip booklet stabilized with plastic backing.
  • Automation – Standardized strips are suitable for automation.

Simple Procedure

  • Identical Serum Dilution (1:100)
  • Internal Test Controls (Serum & Conjugate bands)
  • Quick results (100 minutes: 30/30/10)
+ OVERVIEW

GSD offers both a highly specific line immunoassay (LIA) confirmation test and sensitive ELISA screen to fulfill the CDC’s recommended Lyme disease diagnostic process. The LIA offers numerous improvements compared to traditional Western blot technology.

Line Immunoassay (LIA) vs. Western Blots

Line Immunoassay strips offer easy handling, simple procedures and intuitive interpretation. Results can be read by virtually anyone with minimal training and experience.

Traditional “Western Blots” are not intuitive to read and require a Band Locator to interpret results.

Line Immunoassay (LIA) Advantages

  • Discrete Antigen Application – Antigen applied in tightly defined, discrete positions on each strip.
  • Native Antigen Structure – The 3D (tertiary) structure is maintained for LIA. Western blot proteins are denatured, and only their primary structure remains intact. Antibodies bond with proteins in their native structure.
  • Intuitive Results – Easy-to-read, precisely defined results. No special Band Locater or subjective judgment required.
  • Easy Handling – Strips fixed in a numbered booklet (32 strips per booklet).
  • Durable Strips – Strip booklet stabilized with plastic backing.
  • Automation – Standardized strips are suitable for automation.
+ DETAILS

Simple Procedure

  • Identical Serum Dilution (1:100)
  • Internal Test Controls (Serum & Conjugate bands)
  • Quick results (100 minutes: 30/30/10)

Borrelia burgdorferi B31 Added to CAP Survey

GSD’s Borrelia burgdorferi B31 Line Blots for the confirmation of Lyme disease have been added to the CAP Proficiency Testing Program as part of the Tick-Transmitted Disease (TTD) panel.